Figure 4 - Tissue processing
Shrinkage during processing: the effects of transition solvents. Shrinkage of rat heads fixed in 4% neutral buffered formaldehyde, decalcified in 4 mol/l formic acid, and cleared in methyl salicylate. Measurements were taken at the end of dehydration, clearing and infiltration. Fixed tissue = 100%. Data derived from Brain ¹⁸