Immunohistochemistry Controls for Paraffin Sections

ANTIBODY

TISSUE CONTROL

Actin

Smooth muscle

AA amyloid

AA positive kidney

AL amyloid

AL Kappa or Lambda positive kidney

a -1- antitrypsin

a 1AT deficiency positive liver

a -1-antichymotrypsin

Histiocytoma, tonsil

Alphafetoprotein

Fetal liver

b 2 Microglobulin

Positive & negative synovium

Calcitonin

Thyroid medullary carcinoma

Calretinin

Normal or reactive mesothelium

Carcinoembyonic antigen

Adenocarcinoma colon

CD99/MIC-2

Pancreas

Cerb B2

Breast

Chromogranin

Pancreas

Cytokeratin(MW 52,45/K8,18)

Normal kidney

CMV

CMV infected lung

Desmin

Myometrium (muscle)

EMA

Normal kidney

Estrogen receptor

Breast

Factor VIII

Haemangiopericytoma, tonsil, skin (blood vessels)

Gastrin

Normal gastric antrum

GFAP

Cerebellum

Hepatitis B core ag

Hepatitis B core ag infected liver

Hepatitis B surface ag

Hepatitis B suface ag infected liver

HSV I & II

HSV infected brain

Human placental lactogen

Placenta

HCG (b subunit)

Placenta

HMB45

Melanoma

Keratin MW 56,64/6,9

Skin

Ki67/MIB-2

Tonsil

Lactoferrin

Lactating breast

Lactalbumin

Lactating breast

Leu 7 *CD

Pancreas

Lymphocyte antigens

Tonsil

CD3,CD45,CD20,CD21,CD45RO,CD43,CD45R,CDw75,CD74,CD21,MB2, CD68/KP1

Histiocytoma, spleen

CD15/LeuM1

Hodgkins lymphoma

Lysozyme

Normal duodenum

Myeloperoxidase

Spleen

Myoglobin

Normal skeletal muscle

Neutrophil Elastase

Spleen

Neurofilament

Cerebellum

NSE

Pancreas

Pancreatic hormones

Pancreas

Insulin,Glucagon,Somatostatin

Pancreas

Parathyroid hormone

Parathyroid

Pituitary hormones

Anterior hypophysis

Pneumocystis carinii

Infected lung

Progesterone receptor

Breast

PSA/PAP

Prostate hyperplastic

S100 protein

Melanoma, tonsil

Serotonin

Carcinoid tumour

SP1

Placenta

Thyroglobulin

Thyroid

Vasoactive intestinal peptide

Normal bowel (myenteric plexus)

Vimentin

Myometrium